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In this study, a novel glutathione (GSH) surface molecular imprinting polymer (SMIP) was successfully prepared by using macroporous adsorption resins (MAR) as substrate, which could separate and purify GSH efficiently.

SMIP was synthesized by chloromethylated modified MAR (LX1180-Cl) as the substrate, N, N’-methylenebisacrylamide (NMBA) as a crosslinker, GSH as a template, acrylamide (AM) and N-vinylpyrrolidone (NVP) as functional monomers. The morphology and structure of the polymer were characterized by scanning electron microscope and Fourier transforms infrared spectroscopy.

The maximum adsorption capacity toward GSH was 39.0 mg/g and the separation decree had relation to L-cysteine (L-cys) was 4.2. The optimal operation conditions were studied in detail and the got as follows: the molar ratios of NMBA, AM, GSH and NVP, were 7.0, 0.8 and 0.5. The optimal time and temperature were 14 h and 40°C, respectively. The Langmuir and pseudo-first-order model were fitting these adsorption characteristics well.

GSH has a diversity of medicinal and bioactive functions, so the purpose of this study representing a method in separate and purify technology of GSH, which provided a way for the development of medicine.

This contribution provided a novel way to separate GSH from L-cys. Under the optimal conditions, the maximum adsorption capacity toward GSH was 39.0 mg/g and the separation decree had relation to L-cys was 4.2.

This paper aims to evaluate the separation and purification characteristics of flavonoids from polygonum cuspidatum (PC) extracts by using macroporous adsorption resins (MAR) mixed bed to improve the utilization rate of flavonoids.

Taking the separation performance of flavonoids as an evaluation index, the best MAR were screened from 31 sorts of MAR and combined the best MAR to form a MAR mixed bed for adsorption and separation of flavonoids.

By studying the separation conditions that affect flavonoids, the results showed that resin LZ-72 has best separation and purification effect on flavonoids under the optimal adsorption and desorption conditions, the purity of the obtained flavonoid compound reaches 82.50%, 2.66 times of the initial extract, and the recovery rate reaches 89.70%. Theoretical research results have shown that the adsorption of flavonoids by MAR conforms to the pseudo-second-order kinetics and Freundlich models.

Because the flavonoids in PC have great medicinal value, the purpose of this work is to develop a method of separating and purifying flavonoids from PC, which will provide a certain foundation for the development of medicine.

This contribution provided a novel way to separate flavonoids from PC. Under the optimal conditions, the content of flavonoids in the product was increased 2.66-fold from 31.01% to 82.50%, and the recovery yield was 89.70%.

To save the economic cost and improve the performance of enterprises, this study aims to synthesize high performance immobilized penicillin G acylase (PGA) carriers with fast reaction speed, high recovery rate of enzyme activity and good reusability through corresponding theoretical guidance and experimental exploration.

A diblock resin was synthesized by reversible addition-fragmentation chain transfer polymerization method using N, N-diethylacrylamide (DEA) and β-hydroxyethyl methacrylate (HEMA) as functional monomers poly(N, N-diethylacrylamide)-b-poly(β-hydroxyethyl methacrylate) (PDEA-b-PHEMA) was obtained, and the effect of the ratio of DEA and HEMA on the activity of PGA was investigated, and the appropriate block ratio of DEA and HEMA was obtained. After that, the competitive rate of HEMA and glycidyl methacrylate (GMA) under the carrier preparation conditions was investigated. Based on the above work, a thermosensitive resin carrier PDEA-b-PHEMA-b-P(HEMA-co-GMA) with different target distances was synthesized, and the chemical structures and molecular weight of copolymers were investigated by hydrogen NMR (¹H NMR).

The lower critical solution temperature of the resin support decreases with the increase of the monomer HEMA in the random copolymerization; the catalytic performance study indicated that the response rate of the immobilized PGA is fast, and the recovery rate of the enzyme activity of the immobilized PGA varies with the distance between the targets. When the molar ratio of HEMA to GMA in the resin block is 8.15:1 [i.e. resin PDEA100-b-PHEMA10-b-P(HEMA65-co-GMA8)], the activity recovery rate of immobilized PGA can reach 50.51%, which was 15.49% higher than that of pure GMA immobilized PGA.

This contribution provides a novel carrier for immobilizing PGA. Under the optimal molar ratio, the enzyme activity recovery could be up to 50.51%, which was 15.49% higher than that of PGA immobilized on the carrier with nonregulated distance between two immobilization sites.

A fast algorithm is proposed to calculate the lightning horizontal electric field over a lossy ground.

The lightning horizontal electric field in frequency domain is approximated by a number of piecewise cubic polynomial functions by using the proposed adaptive Hermite strategy. To utilize the Hermite strategy, the frequency domain spectrum and its derivative with respect to frequency are required. The integral kernel of the derivative appears singular along the real axis. To overcome this singularity and accelerate the calculation, a new integration path is proposed. With the help of the Hermite interpolation model and the new path, the lightning horizontal electric field in time domain can be obtained rapidly.

The singularity problem has been overcome with the new integration path and the adaptive Hermite strategy proposed in this paper is at least 50 times faster than the one using the equally spaced sampling approach.

The adaptive Hermite approach can be a good candidate for fitting a wideband frequency domain response and the revised new integration path can be utilized when the calculation of the generalized Sommerfeld integral (GSI) or its derivative with respect to frequency is involved.

Climate change is a massive multidimensional shift. Temperature shifts, in particular, have important implications for urbanization, agriculture, health, productivity, and poverty, among other things. While much research has documented rising mean temperature levels, the authors also examine range-based measures of daily temperature volatility. Specifically, using data for select US cities over the past half-century, the authors compare the evolving time series dynamics of the average daily temperature (AVG) and the diurnal temperature range (DTR; the difference between the daily maximum and minimum temperatures). The authors characterize trend and seasonality in these two series using linear models with time-varying coefficients. These straightforward yet flexible approximations provide evidence of evolving DTR seasonality and stable AVG seasonality.

How do entrepreneurs learn from critical events or significant entrepreneurial experience? It is an important field in entrepreneurship cognition and learning studies. Previous studies have given interpretations from many perspectives, such as effectuation, scenario change of entrepreneurial behaviors, cognitive development, situated learning, etc. These studies provide important clues for exploring the dynamic mechanism of entrepreneurship learning; the problems, such as narrow study angle, fragmentation of knowledge and lack of systematic explanation, however, have also been exposed. For this reason, this study aims to reveal the deep structure and the effectiveness mechanism of entrepreneurs’ learning from critical events, based on existing theoretical progress and specific cases, through the abstract method of retrospective analysis. A conceptual model of entrepreneurial critical events learning is built on this basis, thus deepening the understanding of entrepreneurial learning mechanism.

This study combines retrospection of critical realism with a single case study. On the one hand, data are collected through many channels, such as semi-structured interview, field observation and collection of secondhand information to describe events as detailed as possible. On the other hand, strict coding principles and processes are followed to ensure the validity and reliability of the research.

Entrepreneurial critical event learning reflects the legitimacy, competency and dominancy of entrepreneurial behavior script and leaves a positive influence on the quality improvement of entrepreneurial behavior script. Entrepreneurial critical event learning is an effectual process of decision-making and a process of situated learning and cognitive development. Thus, critical event learning plays an important role in enhancing the influence capacity of stakeholders.

Single case study is used in this paper and, thus, lacks comparison and verification of multi-case study. In addition, selection biases might have occurred during the process of retrospection.

This study broadens research on new enterprise generation process from the aspect of interaction between entrepreneurs and environment. This study reveals the deep structure and effectiveness mechanisms that restrain entrepreneurial behaviors. The study overcomes the problem of over-emphasis on institutional restriction and insufficient understanding on the subjective initiative of entrepreneurs in the research on institutional legitimacy. This study addresses over-emphasis on individual initiative and insufficient focus on behavior legitimacy in effectuation theory.

The authors are committed to providing the Chinese government with a foundation for making decisions that will protect black land and ensure long-term agricultural development.

Using the grounded theory approach, this study investigates the influencing factors affecting the quality of black land in Northeast China and proposes a hypothetical model for the mechanism of the influencing factors on the quality of black land in Northeast China.

The factors influencing the quality of black land include not only soil quality, ecological quality and environmental quality, but also economic quality and management quality, and can be classified into five categories. There are complex influence relationships between various factors and black land quality, with soil quality, ecological quality, environmental quality and management quality having a positive influence on economic quality. Soil quality, ecological quality and environmental quality are all improved as a result of good management. Black land quality is influenced positively by environmental quality, economic quality and management quality.

The quality of black land is a major concern in terms of food production and long-term agricultural development. The black land in Northeast China was chosen as the subject of this study, and the research findings have some limitations. The next step will be to expand from studying the black land in Northeast China to the black land worldwide.

In Northeast China, the quality of the five dimensions of black land must be improved in a coordinated and consistent manner.

This paper aims to focus on the finite element method in the frequency domain (FD-FEM) for the transient electric field in the non-sinusoidal steady state under the non-sinusoidal periodic voltage excitation.

Firstly, the boundary value problem of the transient electric field in the frequency domain is described, and the finite element equation of the FD-FEM is derived by Galerkin’s method. Secondly, the constrained electric field equation on the boundary in the frequency domain (FD-CEFEB) is also derived, which can solve the electric field intensity on the boundary and the dielectric interface with high accuracy. Thirdly, the calculation procedures of the FD-FEM with FD-CEFEB are introduced in detail. Finally, a numerical example of the press-packed insulated gate bipolar transistor under the working condition of the repetitive turn-on and turn-off is given.

The FD-CEFEB improves numerical accuracy of electric field intensity on the boundary and interfacial charge density, which can be achieved by modifying the existing FD-FEMs’ code in appropriate steps. Moreover, the proposed FD-FEM and the FD-CEFEB will only increase calculation costs by a little compared with the traditional FD-FEMs.

The FD-CEFEB can directly solve the electric field intensity on the boundary and the dielectric interface with high accuracy. This paper provides a new FD-FEM for the transient electric field in the non-sinusoidal steady state with high accuracy, which is suitable for combined insulation structure with a long time constant.

To address the problem that the current train operation mode that train selects one of several offline pre-generated control schemes before the departure and operates following the scheme after the departure, energy-saving performance of the whole metro system cannot be guaranteed.

A cooperative train control framework is formulated to regulate a novel train operation mode. The classic train four-phase control strategy is improved for generating specific energy-efficient control schemes for each train. An improved brute force (BF) algorithm with a two-layer searching idea is designed to solve the optimisation model of energy-efficient train control schemes.

Case studies on the actual metro line in Guangzhou, China verify the effectiveness of the proposed train control methods compared with four-phase control strategy under different kinds of train operation scenarios and calculation parameters. The verification on the computation efficiency as well as accuracy of the proposed algorithm indicates that it meets the requirement of online optimisation.

Most existing studies optimised energy-efficient train timetable or train control strategies through an offline process, which has a defect in coping with the disturbance or delays effectively and promptly during real-time train operation. This paper studies an online optimisation of cooperative train control based on the rolling optimisation idea, where energy-efficient train operation can be realised once train running time is determined, thus mitigating the impact of unpredictable operation situations on the energy-saving performance of trains.

This paper aims to investigate the effects of different ultrasonic-assisted loading degrees on the microstructure, mechanical properties and the fracture morphology of Cu/Zn+15%SAC0307+15%Cu/Al solder joints.

A new method in which 45 μm Zn particles were mixed with 15% 500 nm Cu particles and 15% 500 nm SAC0307 particles as solders (SACZ) and five different ultrasonic loading degrees were applied for realizing the soldering between Cu and Al at 240 °C and 8 MPa. Then, SEM was used to observe and analyze the soldering seam, interface microstructure and fracture morphology; the structural composition was determined by EDS; the phase of the soldering seam was characterized by XRD; and a PTR-1102 bonding tester was adopted to test the average shear strength.

The results manifest that Al–Zn solid solution is formed on the Al side of the Cu/SACZ/Al joints, while the interface IMC (Cu₅Zn₈) is formed on the Cu side of the Cu/SACZ/Al joints. When single ultrasonic was used in soldering, the interface IMC (Cu₅Zn₈) gradually thickens with the increase of ultrasonic degree. It is observed that the proportion of Zn or ZnO areas in solders decreases, and the proportion of Cu–Zn compound areas increases with the variation of ultrasonic degree. The maximum shear strength of joint reaches 46.01 MPa when the dual ultrasonic degree is 60°. The fracture position of the joint gradually shifts from the Al side interface to the solders and then to the Cu side interface.

The mechanism of ultrasonic action on micro-nanoparticles is further studied. By using different ultrasonic loading degrees to realize Cu/Al soldering, it is believed that the understandings gained in this study may offer some new insights for the development of low-temperature soldering methodology for heterogeneous materials.